Messages

16

Electronics / Re: Using a sony sensor with the miniscope DAQ?

« on: July 10, 2018, 07:09:39 AM »

Hi,
I haven't had the chance to look over those sensors' data sheets but you will want to find imaging sensors that output an 8 to 12bit parallel bus with clock, line valid, and frame valid control signals all running at under 100MHz. Many large format sensors output over multiple LVDS lines which the rest of the Miniscope electronics cannot handle currently.

17

Data Analysis / Re: error in using etterguillaume/MiniscopeAnalysis

« on: June 18, 2018, 05:28:58 PM »

Hi Xiao,
I think you best bet would be to post this question on their GitHub page. Go to their GitHub repo page and find the "Issues" tab then click "New Issue" and add this information there.

18

Behavior / Re: Behavioral Syncing with TTLs

« on: May 26, 2018, 10:08:52 PM »

Hi Chase,
That feature hasn't been programmed into the DAQ yet but the hardware is capable of doing it. You would need to modify the firmware running on the DAQ and program in this feature. Multiple people have brought up this topic before but I don't know of anyone who actually went into the firmware to add it.

I am currently working on a new DAQ which will have this feature built into it but I imagine the new DAQ won't be ready for prime time till the end of this year at the earliest.

19

DAQ Software/Firmware / Re: msCam Frame retrieve error

« on: May 18, 2018, 04:36:08 PM »

Hi hansu,
Can you try connecting to the Miniscope with a different Windows 10 computer. If the issue you are having persists across multiple Miniscope system then it is likely the issue has something to do with your computer.

The other source of this issue could be if you had a short across the SMA2Coax PCB and then powered on the DAQ PCB. This can cause a power surge across an inductor on the DAQ PCB causing all 3 green LEDs to stay on but the actual data signal being received by the DAQ PCB to be corrupted. If this happened it is pretty easy to fix it by replacing the L13 inductor on the DAQ PCB.

Let me know once you have tried a different computer and if you think there was a chance you had a short on the SMA2Coax PCB at some point in the past.

20

Optics / Re: Miniscope for Bioluminescence imaging

« on: May 02, 2018, 05:45:44 PM »

Hi Dmitrijs,
I don't know of any imaging sensors that might fit the bill while requiring minimum changes to the Miniscope/electronics. Some of the new Miniscope systems we are working on will be moving in this direction to allow for a wider range of imaging sensors and we will be sharing these designs once they are up and running.

You might want to check out what sorts of back illuminated CMOS sensors are on the market right now. Those will be your best bet.

21

Data Analysis / Re: Error in msExtractFiring and additional basic questions for analysis

« on: May 02, 2018, 05:41:18 PM »

Hi kjlawlor,
I can't remember what became of this issue but I suggest you switch over to doing your processing and analysis with the CaImAn package, https://github.com/flatironinstitute/CaImAn. Definitely the standard in the field right now and what we are now using.

22

Behavior / Re: Can I use MiniscopeControl for only behavior video acquisition with trigger

« on: April 27, 2018, 04:34:17 PM »

Hi xiaoyup,
Yes you can use the Miniscope DAQ software for only behavioral cameras. But no, you cannot adjuct behavior video framerate through the software.

23

Electronics / Re: sensor Signal−to−Noise Ratio

« on: April 26, 2018, 04:25:04 PM »

Hi Stephan,
Thanks for posting this information. We have never measured the Photon Transfer Curve ourselves but I will try to get around to doing so soon. Can you upload a plot of your measurements?

24

Optics / Re: grin lens for gofoton

« on: April 22, 2018, 06:04:49 PM »

Hi Richardson,
We have used a bunch of their ~1mm diameter relay lenses which pretty good success. I know multiple groups have been testing their 2mm objective GRIN lenses but we personally haven't had a chance yet.

25

Optics / Re: Miniscope for Bioluminescence imaging

« on: April 22, 2018, 06:02:59 PM »

Hi Dmitrijs,
I haven't heard of anyone doing this yet but it is a really interesting application for Minsicopes. From what I have seen so far with bioluminescence, the MT9V032 likely won't be sensitive enough but there are some much more sensitive CMOS sensors on the market that could fit in the Miniscope form-factor.

26

Data Analysis / Re: Sample Workshop data stats

« on: April 14, 2018, 02:22:07 PM »

Hi and sorry for the issue and delay in responding. The link looks to now be working.

27

DAQ Software/Firmware / Re: which version of visual studio the MiniscopeControl was compiled?

« on: March 28, 2018, 06:04:53 PM »

Hi Richardson,
I wrote the DAQ software using Visual Studio 2012.

28

DAQ Software/Firmware / Re: Capturing miniscope data with micromanager

« on: March 28, 2018, 06:03:59 PM »

Hi,
We haven't tried using micromanager but I think it would be possible. The Miniscope shows up as a generic webcam to the computer so as long as micromanager can stream in a webcam video (using the standard UVC protocol) then it should work.

29

DAQ Software/Firmware / Re: Running 2 DAQ Boards on the same Workstation

« on: March 28, 2018, 06:01:42 PM »

Hi Ivo,
As long as your USB3 driver and computer hardware can handle the bandwidth of 2 Miniscopes you should be able to run 2 at the same time on the same computer.

30

DAQ Software/Firmware / Re: Error recognizing USB. Install failed.

« on: March 28, 2018, 05:55:54 PM »

Hi Phil,
From what I understand LabMaker tests their boards before shipping them out and has already performed the EEPROM programming through the board. You should contact them to double check that this is the case and that they were able to get it to function properly.

The soldering looks alright to me. I would suggest buying a couple different USB cables first and see if that is the issue. Are you running Windows 7 through Bootcamp on your Mac? Parallels won't work.