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**Data Analysis / msRun Error**

« **on:**February 28, 2018, 06:44:49 PM »

Hi,

I've been trying to do analysis through the pipeline. And after aligning, the error message is keeping popped up. I am pretty newbie in the Matlab, so I couldn't make any change with this error.

>> msRun

Calculating column correction. 12.5313% done

Calculating column correction. 75.1876% done

Calculating fluorescence properties: 25% done.

Calculating fluorescence properties: 50% done.

Calculating fluorescence properties: 75% done.

Use mouse to select threshold for mean good fluorescence value

Keep selection? (Y/N)y

Select ROI #1

Select another ROI? (Y/N)Y

Select ROI #2

Select another ROI? (Y/N)N

Alignment 1/2

Calculating shift between frames. On frame 1000

Calculating shift between frames. On frame 2000

Calculating shift between frames. On frame 3000

Alignment 2/2

Calculating shift between frames. On frame 1000

Calculating shift between frames. On frame 2000

Calculating shift between frames. On frame 3000

Elapsed time is 795.770971 seconds.

Select best alignment (Enter number):1

Reading video into memory. 12.5313% done

Reading video into memory. 75.1876% done

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

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Could not find bounds

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Could not find bounds

Could not find bounds

Extracting dF/F traces: 5.0025

Extracting dF/F traces: 10.005

Extracting dF/F traces: 15.0075

Extracting dF/F traces: 20.01

Extracting dF/F traces: 25.0125

Extracting dF/F traces: 30.015

Extracting dF/F traces: 35.0175

Extracting dF/F traces: 40.02

Extracting dF/F traces: 45.0225

Extracting dF/F traces: 50.025

Extracting dF/F traces: 55.0275

Extracting dF/F traces: 60.03

Extracting dF/F traces: 65.0325

Extracting dF/F traces: 70.035

Extracting dF/F traces: 75.0375

Extracting dF/F traces: 80.04

Extracting dF/F traces: 85.0425

Extracting dF/F traces: 90.045

Extracting dF/F traces: 95.0475

Reference to non-existent field 'vidObj'.

Error in msGenerateVideoObj (line 33)

ms.height = ms.vidObj{1}.Height;

Error in msRun (line 78)

behav = msGenerateVideoObj(pwd,'behavCam');

>>

How can we fix this?

I've been trying to do analysis through the pipeline. And after aligning, the error message is keeping popped up. I am pretty newbie in the Matlab, so I couldn't make any change with this error.

>> msRun

Calculating column correction. 12.5313% done

Calculating column correction. 75.1876% done

Calculating fluorescence properties: 25% done.

Calculating fluorescence properties: 50% done.

Calculating fluorescence properties: 75% done.

Use mouse to select threshold for mean good fluorescence value

Keep selection? (Y/N)y

Select ROI #1

Select another ROI? (Y/N)Y

Select ROI #2

Select another ROI? (Y/N)N

Alignment 1/2

Calculating shift between frames. On frame 1000

Calculating shift between frames. On frame 2000

Calculating shift between frames. On frame 3000

Alignment 2/2

Calculating shift between frames. On frame 1000

Calculating shift between frames. On frame 2000

Calculating shift between frames. On frame 3000

Elapsed time is 795.770971 seconds.

Select best alignment (Enter number):1

Reading video into memory. 12.5313% done

Reading video into memory. 75.1876% done

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Could not find bounds

Extracting dF/F traces: 5.0025

Extracting dF/F traces: 10.005

Extracting dF/F traces: 15.0075

Extracting dF/F traces: 20.01

Extracting dF/F traces: 25.0125

Extracting dF/F traces: 30.015

Extracting dF/F traces: 35.0175

Extracting dF/F traces: 40.02

Extracting dF/F traces: 45.0225

Extracting dF/F traces: 50.025

Extracting dF/F traces: 55.0275

Extracting dF/F traces: 60.03

Extracting dF/F traces: 65.0325

Extracting dF/F traces: 70.035

Extracting dF/F traces: 75.0375

Extracting dF/F traces: 80.04

Extracting dF/F traces: 85.0425

Extracting dF/F traces: 90.045

Extracting dF/F traces: 95.0475

Reference to non-existent field 'vidObj'.

Error in msGenerateVideoObj (line 33)

ms.height = ms.vidObj{1}.Height;

Error in msRun (line 78)

behav = msGenerateVideoObj(pwd,'behavCam');

>>

How can we fix this?