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Topics - hsiao

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1
DAQ Software/Firmware / Event marker
« on: March 10, 2019, 03:18:24 PM »
Hi,

I am wondering if the elapsedTime in settings_and_notes.dat can be millisecond since I was using the note function as an event marker for stimuli. A second resolution is not enough for this.

Any suggestions?

Thank you!

hsiao 

2
General Discussion / Happy New Year 2019
« on: January 01, 2019, 04:26:03 AM »
Hello everyone,

Wish everybody has remarkable success in your experiments.

I'll keep working on Ca imaging this year, too.

I am curious about the difficulty of seeing Ca imaging in mice.
Does anyone like to talk about your percentage of successful seeing signal?

I am especially interested in the dorsal, ventral hippocampus and the amygdala.

I hope someone can give me some directions.


Thanks!

3
General Discussion / Virus injection and lens implantation
« on: August 16, 2018, 08:45:06 AM »
Hi,

I heard some researchers inject the virus and wait for ~2 weeks (or longer) then implant lens and wait for another week.

But the protocols from Shanna et. al. recommend combining them into one surgery.


Does anyone know the pros and cons of doing these protocols separately?

Thank you!

Hsiao

4
Surgery and Baseplating / nothing but gray
« on: July 13, 2018, 06:28:56 AM »
Hi,

I was trying to do my first Gcamp experiment.

I planned to record dCA1 in rats. Since the grin lens (#64-519, Edmund optics) is not long enough, I stacked 2 lenses and screwed them in the bottom of Miniscope as an object lens. Then, I injected AAV into the dCA1 around the stratum radiatum and implanted 1 lens on the pyramidal layer (total 3 lenses, including 2 object and 1 relay lenses). After 3 weeks, I saw nothing. I even canít see the blood vessels. I could only see black and gray under the maximal power and gain. I waited another week but still had no luck. You can check the screenshot below. This is a screenshot after 4 weeks of incubation.

 
I just perfused this rat, no obvious blood clot is noticed. I am still waiting for the histology and want to make sure my Gcamp express well but canít wait to ask questions here for improving the protocol for my second rat.

 

I am not sure if my setup is correct (2 object lenses plus 1 relay lens) but I did test and see clear signals on a paper with green lines. I used some methods like this http://miniscope.org/index.php/Initial_Testing_of_Assembled_Miniscopes

 

Thank you!


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