Miniscope Board

General Category => Optics => Topic started by: chalspark on November 28, 2016, 08:44:52 AM

Title: Calculate magnification
Post by: chalspark on November 28, 2016, 08:44:52 AM

Dear all,

Hello. Is anyone can calculate magnification? or um per pixel?

we used 1.8mm Dia, 670nm DWL, 0.0mm WD, Uncoated, GRIN Lens. (http://www.edmundoptics.com/optics/optical-lenses/aspheric-lenses/gradient-index-grin-rod-lenses/64519/)

In web site, magnification is x6 to x7 and ~1um per pixel.

How can i calculate these value?

It will be help for scale bar into image.

Thanks.

Title: Re: Calculate magnification
Post by: Daniel Aharoni on November 28, 2016, 11:48:30 PM

Hi Chalspark,
Can you tell me which focal length achromatic lens you are using? Once I have the info I can simulate your system to give you an approximate magnification. Ideally you should use a resolution calibration slide with some green fluorescent tape under it to directly measure the magnification of your system since there may be small differences in magnification between systems due to how the scope was assembled.

Title: Re: Calculate magnification
Post by: chalspark on November 29, 2016, 02:03:09 AM

Hi Daniel,

We used 5mm Dia. x 12.5mm FL, MgF2 Coated, Achromatic Doublet Lens.

Could you tell me formula in order to calculate magnification?

Thanks.